Phd position off-gas spectroscopy for physiology and process biomarker

PhD Position Off-Gas Spectroscopy for Physiology and Process Biomarker Job description

Collaboration

This PhD project is part of a collaboration between Delft University of Technology (TU Delft) and University of Colorado Boulder (CU Boulder). The partnership includes three TU Delft research groups at the Department of Biotechnology (dr. Klijn, dr. Vieira-Lara, and dr. Jourdin) and one CU Boulder research group embedded in the Department of Mechanical Engineering (prof. Rieker). The overarching aim of this collaboration is to integrate and apply a novel analytical technique to perform real-time spectroscopic monitoring of the bioreactor off-gas for two different types of bioprocesses, namely microbial fermentation in suspension and biofilm-based microbial electrosynthesis. With this technology we set out to advance bioprocess understanding and accelerate bioprocess optimization, scale-up, and automated control.

Team

The content of the partnership leads to highly interdisciplinary and collaborative research. The complete team to be implemented at TU Delft will consist of 2 post-doc positions (1. Quantitative monitoring of MES and 2. Quantitative monitoring of microbial suspension cultures), 2 PhD candidates (1. Volatile fingerprints of microbial suspension cultures and 2. Data analytics/machine learning and control), and 2 technicians (1. Support MES cultures and 2. Support suspension cultures, both are involved with tech transfer and integration). In addition, there are three research positions part of prof. Rieker's team at CUB with which we will closely collaborate throughout the project. We are looking for people who can showcase their ability to work well in a team, are effective in sharing theoretical knowledge and technical know-how, and excel as creative problem solvers.

PhD project

The PhD position in this vacancy will focus on the discovery of novel volatile bioprocess biomarkers using (near) real-time novel spectroscopic off-gas techniques. In this project, you will have the opportunity to work with yeast bioreactors and explore the volatile compounds that are produced under different growth conditions. Process heterogeneity studies will be conducted at lab scale (scale-down) to identify novel bioprocess biomarkers. Orthogonal monitoring of physiology (e.g. Transcriptomics and Raman) will be conducted in parallel with the aims of (1) better understanding cell physiology and (2) aiding biomarker identification. Finally, you will have the opportunity to test and validate biomarkers in different culture setups.

The PhD position co-supervised by dr. Vieira-Lara and dr. Klijn.

The group led by dr. Vieira-Lara is called Bioenergetics and Quantitative Biology. We investigate metabolic strategies of industrially relevant organisms from a quantitative perspective, combining fundamental research with applied approaches to enhance industrial processes.

The group led by dr. Klijn is called: Data-Driven Bioprocess Development group. In this group, we work on a wide variety of biotechnological processes (microbial, mammalian, ATMP) and the implementation of process analytical technology (such as FT-IR and Raman spectroscopy) for process development and process automated control purposes.

Job requirements

This project is experimental with strong emphasis on analytics, but also on (omics) data analysis. We are looking for an outstanding and creative researcher with affinity for interdisciplinary projects. In this project you will have the opportunity to work with cell physiology, bioreactors, off-gas measurement analytics, and omics in a highly collaborative team. We are looking for a candidate with the motivation to expand their horizon, able to learn quickly, and who has great communication skills to easily connect and collaborate with all team members. This project will be supported by a technician who will work closely with other team members.

Required educational background

• A MSc degree in biotechnology, microbiology, (analytical) biochemistry or a closely related discipline

Recent experience or clearly supported motivation

• Recent experience with microbial fermentation or cell culture is considered a must
• Strong motivation for learning or recent experience with novel analytical technology is considered essential
• Recent experience in data analysis

Required technical skills or strong motivation to learn

• Bioreactor cultivation
• Analysis of large datasets
• Analytical techniques (HPLC, GC-MS, etc)
• Programming

Sought-after transferable skills

• Pro-active and problem solving mindset
• Excellent written and spoken English skills
• Ability to break-down and communicate complex research data
• Thrives in a fast-paced, multidisciplinary, and highly collaborative environment

TU Delft (Delft University of Technology)

Delft University of Technology is built on strong foundations. As creators of the world-famous Dutch waterworks and pioneers in biotech, TU Delft is a top international university combining science, engineering and design. It delivers world class results in education, research and innovation to address challenges in the areas of energy, climate, mobility, health and digital society. For generations, our engineers have proven to be entrepreneurial problem-solvers, both in business and in a social context.

At TU Delft we embrace diversity as one of our core values and we actively engage to be a university where you feel at home and can flourish. We value different perspectives and qualities. We believe this makes our work more innovative, the TU Delft community more vibrant and the world more just. Together, we imagine, invent and create solutions using technology to have a positive impact on a global scale. That is why we invite you to apply. Your application will receive fair consideration.

Challenge. Change. Impact!

Faculty Applied Sciences

With more than 1,100 employees, including 150 pioneering principal investigators, as well as a population of about 3,600 passionate students, the Faculty of Applied Sciences is an inspiring scientific ecosystem. Focusing on key enabling technologies, such as quantum- and nanotechnology, photonics, biotechnology, synthetic biology and materials for energy storage and conversion, our faculty aims to provide solutions to important problems of the 21st century. To that end, we educate innovative students in broad Bachelor's and specialist Master's programmes with a strong research component. Our scientists conduct ground-breaking fundamental and applied research in the fields of Life and Health Science & Technology, Nanoscience, Chemical Engineering, Radiation Science & Technology, and Engineering Physics. We are also training the next generation of high school teachers.

Click here to go to the website of the Faculty of Applied Sciences.

Conditions of employment
Doctoral candidates will be offered a 4-year period of employment in principle, but in the form of 2 employment contracts. An initial 1.5 year contract with an official go/no go progress assessment within 15 months. Followed by an additional contract for the remaining 2.5 years assuming everything goes well and performance requirements are met.

Additional information
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Application procedure
3 February 2025

• 2-page CV, including contact details of 2 to 3 references
• 1-page motivation letter

If your application package does not include these items, we cannot take your application under consideration.

Doing a PhD at TU Delft requires English proficiency at a certain level to ensure that the candidate is able to communicate and interact well, participate in English-taught Doctoral Education courses, and write scientific articles and a final thesis. For more details please check the Graduate Schools Admission Requirements.